Anti-alpha Tubulin antibody [B-5-1-2] ab11304 from Abcam

Product Specs
ItemAnti-alpha Tubulin antibody [B-5-1-2]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab11304
Domain/Region/Terminus5-1
Antibody TypePrimary
IsotypeIgG1
ClonalityMonoclonal
HostMouse, Nude mouse
Quantity50 UL
ApplicationsWestern blot
CloneB-5-1-2
Conjugate/TagUnconjugated
Concentration2.3 mg/ml
Gene NameTUBA4A
ReactivityHuman
Targetalpha Tubulin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent agingEMBO reportsDecember 6, 2023Monami Ogura, Tatsuya Kaminishi, Takayuki Shima, Miku Torigata, Nao Bekku, Keisuke Tabata, Satoshi Minami, Kohei Nishino, Akiko Nezu, Maho Hamasaki, et al.anti‐GFP (rabbit, 1/1,000; Cell Signaling Technology, 2555), anti‐α‐tubulin (mouse, 1/10,000; Abcam, ab11304), anti‐EGFR (sheep, 1/1,000; Fitzgerald, 20‐ES04), anti‐Atg5 (rabbit, 1/1,000; Cell Signaling Technology,

(2) The crosstalk between glomerular endothelial cells and podocytes controls their responses to metabolic stimuli in diabetic nephropathyScientific ReportsOctober 20, 2023Michael Albrecht, Carsten Sticht, Tabea Wagner, Steffen A. Hettler, Carolina De La Torre, Jiedong Qiu, Norbert Gretz, Thomas Albrecht, Benito Yard, Jonathan P. Sleeman, et al.ascites, final dilution 1:20,000–1:50,000), α-tubulin mouse monoclonal antibody (Abcam, clone B-5-1-2, ab11304, final concentration 0.05 µg/mL). After washing 3 times with TBST (50 mM Tris, 146 mM NaCl, 0.1% Tween 20

(3) A non‐translational role of threonyl‐tRNA synthetase in regulating JNK signaling during myogenic differentiationThe FASEB JournalMay 17, 2023Chong Dai, Adriana Reyes‐Ordoñez, Jae‐Sung You, Jie ChenSciences). Anti-Flag-BioM2 (F9291), anti-MEKK4 (M7194), and gelatin were purchased from Sigma. Anti-tubulin (ab11304) and anti-ThrRS (ab236903) were from Abcam. Antibodies for the following proteins were obtained from

(4) Substrate-driven assembly of a translocon for multipass membrane proteinsNatureNovember 1, 2022Sundaram A, Yamsek M, Zhong F, Hooda Y, Hegde RS, Keenan RJ.(PA5-21773; 1:1,000) from Invitrogen; rabbit anti-uL22 from Abgent (AP9892b; 1:1,000); mouse anti-tubulin (ab11304; 1:1,000) and mouse anti-HRP (ab6728; 1:1,000) from Abcam; mouse anti-Flag (F1804; 1:1,000), rabbit

(5) MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian InsufficiencyCellsSeptember 7, 2022Zobia Umair, Mi-Ock Baek, Jisue Song, Seona An, Seung Joo Chon, Mee-Sup YoonImageJ software. The following antibodies were used: anti-TSG101 (ab125011, 1:1000) and anti-tubulin (ab11304, 1:2000) from Abcam (Cambridge, MA, USA); anti-CD9 (sc-13118, 1:200), anti-CD63 (sc-5275, 1:200), anti-STAT3

(6) C-Peptide Promotes Cell Migration by Controlling Matrix Metallopeptidase-9 Activity Through Direct Regulation of β-Catenin in Human Endometrial Stromal CellsFrontiers in Cell and Developmental BiologyJanuary 21, 2022Sana Abdul Khaliq, Zobia Umair, Mi-Ock Baek, Seung Joo Chon, Mee-Sup Yoon(13667), and PP2A (2259) were obtained from Cell Signaling Technology. Antibodies against tubulin (ab11304) and PP1A (MAB3000) were purchased from Abcam and R&D Systems (Minneapolis, MN, United States), respectively.

(7) Cervical carcinoma high-expressed long non-coding RNA 1 promotes papillary thyroid carcinoma cell proliferation and invasionTranslational Cancer ResearchSeptember 1, 2021Ping Shi, Yan Liu, Mingzeng Zhang, Jianwang Yang, Shanghua Jing, Dongqiang Yang, Fei Liu, Yanzhao Wu, Huijing Shi, Cuizhi Genganti-p-ERK (ab176660), anti-p38 MAPK (ab197348), anti-p-p38 MAPK (ab176664), and anti-microtubule protein (ab11304) (Abcam, USA). Then, the membranes were incubated with a 1:2,000 dilution of anti-rabbit or anti-mouse

(8) Molecular Mechanisms Underlying the Acclimation of Chlamydomonas reinhardtii Against Nitric Oxide StressFrontiers in Plant ScienceAugust 5, 2021Eva YuHua Kuo, Tse-Min Leeanti-DHAR (LKT BioLaboratories Ltd., Taoyuan, Taiwan), or a mouse monoclonal antibody against α-tubulin (ab11304; Abcam, Cambridge, United Kingdom). Following the incubation with horseradish peroxidase-conjugated

(9) Development of a Specific Monoclonal Antibody to Detect Male Cells Expressing the RPS4Y1 ProteinInternational journal of molecular sciencesFebruary 18, 2021Spena S, Cordiglieri C, Garagiola I, Peyvandi F.(MA5-15123; Invitrogen, Waltham, MA, USA) (1:1000) and the anti-alpha tubulin monoclonal antibody (ab11304; Abcam, Cambridge, UK) (1:6000) were used as positive controls. After washing, the membranes were incubated

(10) Muscle-derived TRAIL negatively regulates myogenic differentiationExperimental cell researchSeptember 1, 2020Kim D, Singh N, Waldemer-Streyer RJ, Yoon MS, Chen J.antibody (A-11072, 1:500) and U0126 were from Thermo Fisher Scientific (Waltham, MA, USA). Anti-tubulin (ab11304, 1:10000) and anti-p21 (ab109199, 1:500) were from Abcam (Cambridge, MA, USA). Anti-TRAIL-R2 (AF721,

(11) Drp1 overexpression induces desmin disassembling and drives kinesin-1 activation promoting mitochondrial trafficking in skeletal muscleCell death and differentiationAugust 1, 2020Giovarelli M, Zecchini S, Martini E, Garrè M, Barozzi S, Ripolone M, Napoli L, Coazzoli M, Vantaggiato C, Roux-Biejat P, et al.which were purchased from Santa Cruz Biotechnology (Dallas, TX, USA); antibodies against alpha-Tubulin (ab11304-100; IF/PLA 1:100), KIF5B (ab167429; WB 1:1000, IF/PLA 1:200, IP 1:100), and KLC1 (ab174273; IF/PLA

(12) Unbiased yeast screens identify cellular pathways affected in Niemann-Pick disease type CLife science allianceJuly 1, 2020Colaco A, Fernández-Suárez ME, Shepherd D, Gal L, Bibi C, Chuartzman S, Diot A, Morten K, Eden E, Porter FD, et al.mouse anti-acetylated α-tubulin (6-11B-1; Santa Cruz Biotechnology), mouse monoclonal anti–α-tubulin (ab11304; Abcam). Quant-iT PicoGreen sdDNA (Thermo Fisher Scientific) tetramethylrhodamine methyl ester (TMRM;

(13) High Light-Induced Nitric Oxide Production Induces Autophagy and Cell Death in Chlamydomonas reinhardtiiFrontiers in Plant ScienceJune 10, 2020Eva YuHua Kuo, Hsueh-Ling Chang, Shu-Tseng Lin, Tse-Min Leeantibody (ab77003; Abcam, Cambridge, United Kingdom) and a mouse monoclonal antibody against α-tubulin (ab11304; Abcam, Cambridge, United Kingdom) for 90 min at room temperature. After being washed, the membranes

(14) Three-Component Repurposed Technology for Enhanced Expression: Highly Accumulable Transcriptional Activators via Branched Tag ArraysThe CRISPR JournalApril 26, 2019Atsushi Kunii, Yoshihiro Hara, Mitsumasa Takenaga, Naoko Hattori, Takuya Fukazawa, Toshikazu Ushijima, Takashi Yamamoto, Tetsushi Sakumaendogenous protein detection, anti-E-cadherin (ab40772; Abcam, Cambridge, United Kingdom) or anti-α-tubulin (ab11304; Abcam); for MS2-22sTag protein detection, anti-HA (ab49969; Abcam) at a 1:1,000 (ab40772 and ab11304)

(15) Proteasomal degradation of BRAHMA promotes Boron tolerance in ArabidopsisNature communicationsDecember 11, 2018Sakamoto T, Tsujimoto-Inui Y, Sotta N, Hirakawa T, Matsunaga TM, Fukao Y, Matsunaga S, Fujiwara T.Laboratories, Kokubunji, Tokyo, Japan) (1:1000 dilution), anti-RPT350 (1:5000 dilution), anti-α-tubulin (ab11304, Abcam, Cambridge, MA, USA) (1:5000 dilution), anti-histone H3 (MABI0301, MBL) (1:2000 dilution), anti-acetylated

(16) FEZF1在人胚胎干细胞早期神经分化过程中的作用研究SCIENTIA SINICA VitaeMarch 1, 2018培苏, 洪涛王, 利莎卢, 家喜周, 鑫刘, 子岑冯(I) OCT3/4 Santa Cruz SC-9081 1:200 (W) NANOG Cell Signaling techonology #3508 1:1000 (W) α-Tublin Abcam ab11304 1:10000 (W) GAPDH Cell Signaling #2118 1:1000 (W) a) I: 免疫荧光染色; W: 蛋白质免疫印迹 1.7 免疫荧光染色 DMEM/F12培养基中进行分化,

(17) Ampk phosphorylation of Ulk1 is required for targeting of mitochondria to lysosomes in exercise-induced mitophagyNature CommunicationsDecember 1, 2017Rhianna C. Laker et al.

(18) Cxcl14 depletion accelerates skeletal myogenesis by promoting cell cycle withdrawalNPJ Regenerative medicineJanuary 1, 2017Waldemer-Streyer RJ, Reyes-Ordoñez A, Kim D, Zhang R, Singh N, Chen J.Novus Biologicals (Littleton, CO, USA). Anti-F4/80 (MCA497RT) was from Bio-Rad (Hercules, CA, USA). Anti-tubulin (ab11304) was from Abcam (Cambridge, MA, USA). Anti-p21 (sc-471:M-19) was from Santa Cruz Biotechnology (Dallas,

(19) The Mitochondrial Permeability Transition Pore Regulator Cyclophilin D Exhibits Tissue-Specific Control of Metabolic HomeostasisPLOS ONEDecember 22, 2016Rhianna C. Laker, Evan P. Taddeo, Yasir N. Akhtar, Mei Zhang, Kyle L. Hoehn, Zhen Yanab5432), mouse anti-phospho-Akt S473 (CST, #4051), rabbit anti-Akt (CST, #4691), mouse α-Tubulin (Abcam, ab11304). Secondary antibodies were goat anti-mouse IR680 (LICOR) and goat anti-rabbit IR800 (LICOR). Membranes

(20) PLD1 regulates adipogenic differentiation through mTOR - IRS-1 phosphorylation at serine 636/639Scientific reportsNovember 22, 2016Song HI, Yoon MS.reagents Antibodies against the following proteins were obtained from the following sources: alpha-tubulin (ab11304) from Abcam; DEPTOR (NBP149674) from Novus Biologicals; raptor (for IP, A300-553A) from Bethyl laboratories;

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Anti-alpha Tubulin antibody [B-5-1-2] from Abcam

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Anti-alpha Tubulin antibody [B-5-1-2] from Abcam

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